Our company:
Pampa Biotech Spa. is located in the city of Antofagasta, northern Chile. Our mission is to develop products and teaching aid tools derived from the knowledge of molecular biology, microbiology and synthetic biology of extremophiles microorganism of the Atacama Desert.
Our team has a high level of knowledge and expertise in the molecular biology and microbiology of extremophiles microorganism and we have strategic partners in different productive and commercial areas that allow us to offer our clients the best selection of products and services.
Founding Partners:
| Partner | Expertise | % of shares and main role |
|---|---|---|
| Bernardita Valenzuela G. Dr. Applied Sciences |
Microbiology of extreme environments | 50% shares General Manager (CEO) |
| Francisco Solís C. Dr. Master in Applied Sciences |
Molecular Tool Design | 40% shares Operation Manager (COO) |
| Pedro Zamorano M. PhD. Endocrinology |
Synthetic Biology and Molecular Biology | 10% shares Research and Development Manager |
Our company has 3 main divisions:
Education and Science Learning (Biohackers)
This division develops molecular biology and microbiology kits to teach sciences to students in primary and secondary education.
Division of Research and Molecular Design
This division provides gene design services and gene construction for the expression of recombinant genes, the creation of new controlled expression systems and innovative biotechnological tools for industrial applications.
Biochemistry and Molecular Biology Consulting
Science and biotechnology consulting services to industry and educations companies.
Cellular and Molecular Design for Innovation
Education and Science Learning
Kits for Education Sciences
- Microbiology
- DNA extraction
- PCR
- Cloning
- Enzymology
- Recombinant expression
- Buffer solutions and standards
Research Molecular Design
- Recombinant expression systems
- Synthetic biology
- Cloning and expression
Biochemistry and Molecular Biology Consulting
- Consulting services to industry and educations companies
Education and Science Learning
Kits for Education Sciences
- Cat. No. BHK001: Discovering DNA
- Cat. No. BHK002: Knowing the microorganisms
- Cat. No. BHK003: Bacterial transformation
- Cat. No. BHK004: Plasmid extraction and restriction tests
- Cat. No. BHK005: Protein electrophoresis, recombinant expression
- Cat. No. BHK006: Enzymatic assays
- Cat. No. BHK007: Polymerase chain reaction (PCR)
- Cat. No. BHK008: Plant cloning
Cat. No. BHK001: Discovering DNA
- Lisis solution
- Protein precipitation solution
- DNA precipitation solution
- Sterile micro tubes
- Nuclease free tips
- Waste disposal bag
- Waste removal service
Requiered equipment:
- Dry bath (30-90ºC)
- Centrifuge
- Horizontal electrophoresis kit
Cat. No. BHK002: Knowing the Microorganisms
- Sterile liquid culture medium
- Semi-solid culture medium in Petri dishes
- Positive control bacteria
- Sterile conical tubes
- Sterile torulas
- Waste bag
Requiered equipment:
- Dry bath (30-90ºC)
- Centrifuge
- UV light flashlight
Cat. No. BHK003: Bacterial Transformation
- Plasmids
- Culture medium + Antibiotic
- Bacteria
- Sterile conical tubes
- Sterile handles
- Waste bag
Requiered equipment:
- Dry bath (30-90ºC)
- Centrifuge
- UV light flashlight
Cat. No. BHK004: Plasmid Extraction and Restriction Tests
- Plasmids
- Culture medium + Antibiotic
- Bacteria
- Restriction enzymes
- Sterile conical tubes
- Sterile torulas
- Waste bag
Requiered equipment:
- Dry bath (30-90ºC)
- Centrifuge
- UV light flashlight
Cat. No. BHK005: Protein Electrophoresis, Recombinant Expression
- Bacteria with recombinant proteins
- Sterile tubes
- Culture handles
- Waste bag
- Culture medium with inductor
Requiered equipment:
- Vertical electrophoresis set
- Power supply
- Dry bath (30-90ºC)
- Centrifuge
Cat. No. BHK006: Enzymatic Assays
- Enzymatic solution
- Substratum
- Standard solution
- Developer solution
- Petri dishes with agar substrate
- Sterile tubes
Requiered equipment:
- Timed dry bath (30-90ºC)
- Incubator
Cat. No. BHK007: Polymerase Chain Reaction (PCR)
1. Reagents
- Primers
- Standard DNA
- Master Mix (Taq Pol, dNTPs, MgCl2, buffer)
- DNA Ladder
- Agarose
- Charge Buffer 5X
- TAE 10X Buffer
- H2O Nuclease free
- Microtubes (0,2/0,5/1,5 ml)
2. Equipment
Thermocycler, Eletrophoresis, Transliluminator UV, Centrifuge, Micropipetes
3. Training
Cat. No. BHK008: Plant Cloning
- Sterile tubes with culture medium
- Set of materials for cutting and handling
- Phytohormones solution
Requiered equipment:
- Daylight lamp
- Automatic programmer for daylight hours
Biochemistry and Molecular Biology Consulting
Talks and Presentations
- Cat. No. BH001: Synthetic Biology
- Cat. No. BH002: Biotechnological Potential of Thermophilic Microorganisms
- Cat. No. BH003: Biotechnological Applications to Industry
- Cat. No. BH004: Astrobiology and Atacama Desert
- Cat. No. BH005: Climate Change and Microorganisms
- Cat. No. BH006: Astrobiology and Evolution
- Cat. No. BH007: Hi, I'm a bacterium
- Cat. No. BH008: Thermus thermophilus: molecular biology study model
- Cat. No. BH009: Thermophilic microorganisms: The Hot side of evolution
Courses
- Cat. No. BC001: Synthetic Biology
- Cat. No. BC002: Biotechnological Potential of Thermophilic Microorganisms
- Cat. No. BC003: Biotechnological Applications to Industry
- Cat. No. BC004: DNA: Information and manipulation
- Cat. No. BC005: Recombinant Proteins
- Cat. No. BC006: RNA: information and manipulation
- Cat. No. BC007: Basic Microbiology
- Cat. No. BC008: Molecular Biology for non-biologists
- Cat. No. BC009: Extremophilic microorganisms of the Antofagasta region, basic course for tourist guides
- Cat. No. BC010: Implementation of a basic molecular biology laboratory for middle school students
- Cat. No. BC011: Microbiology of Extreme Environments, basic course for communities of native people
Reagents and Laboratory Solutions
- Cat. No. SR001: TAE 50X Buffer, 30 ml
- Cat. No. SR002: PBS Buffer, pH 8, 10X, 30 ml
- Cat. No. SR003: TE buffer, pH 8, 20 ml
- Cat. No. SR004: Ampicillin 10X, 1 ml
- Cat. No. SR005: Kanamycin 10X, 1ml Cat. No. SR006: Lysozyme 10X, 1 ml
- Cat. No. SR007: RNAse 10X, 1 ml
- Cat. No. SR008: Molecular weight marker, 120 ul
- Cat. No. SR009: 0.5 M KCl solution, 50 ml
- Cat. No. SR010: Iodine solution, 50 ml
- Cat. No. SR011: 70% Ethanol, 300 ml
- Cat. No. SR012: Absolute ethanol, molecular grade, 40 ml
- Cat. No. SR013: Set of Gram staining solutions (4), 20 ml
Culture Media
- Cat. No. M001: Medium LB, liquid 200 ml
- Cat. No. M002: Liquid medium for fungal culture, 100 ml
- Cat. No. M003: Medium LB, agar 200 ml
- Cat. No. M004: Petri dishes with LB / agar medium, 10 units
- Cat. No. M005: Petri dishes with LB medium / agar + ampicillin, 10 units
- Cat. No. M006: Petri dishes with LB medium / agar + ampicillin + IPTG + X-GAL, 10 units
Other Reagents
- Cat. No. CC01: Chemo-competent cells (-80ºC), 2 u
- Cat. No. CC02: Chemo-competent cells (-80ºC), PK6
- Cat. No. CC03: Chemo-competent cells (-80ºC), 12 pcs
Consulting Services
- Design of specific and/or degenerated primers
- DNA segment amplification (PCR)
- Cloning
- Preparation of applied research projects
- Microbiological monitoring of aquatic ecosystems